Hotspot

trnfM-CAU-rps14

Genome · 43661-43769 · 109 bp

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Pi 0.138
MarkerSeek score 78.60
Primer available yes
Label trnfM-CAU-rps14

Feature Metadata

label_name
trnfM-CAU-rps14
feature_type
igs
region
Genome
length_bp
109
variable_sites
15
pi
0.138
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-rps14_p1 GAGCTACCAAACCGCTCTAC ACCGGGAGTCACTAAATCGA 55.0 50.0 58.1 58.2 163-165 bp 1.0 85.5
trnfM-CAU-rps14_p2 AAGGTTATGAGCCTTGCGAG ACCGGGAGTCACTAAATCGA 50.0 50.0 58.0 58.2 180-182 bp 1.0 85.1
trnfM-CAU-rps14_p3 GTTATGAGCCTTGCGAGCTA ACCGGGAGTCACTAAATCGA 50.0 50.0 57.8 58.2 177-179 bp 1.0 83.7
trnfM-CAU-rps14_p4 TTATGAGCCTTGCGAGCTAC ACCGGGAGTCACTAAATCGA 50.0 50.0 57.8 58.2 176-178 bp 1.0 83.7
trnfM-CAU-rps14_p5 CGATTTCCTATGGCGGAGAC ACCGGGAGTCACTAAATCGA 55.0 50.0 58.2 58.2 221-223 bp 1.0 82.4