Hotspot

cysA-trnT-GGU

Genome · 38701-39452 · 752 bp

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Pi 0.112
MarkerSeek score 76.00
Primer available yes
Label cysA-trnT-GGU

Feature Metadata

label_name
cysA-trnT-GGU
feature_type
igs
region
Genome
length_bp
752
variable_sites
61
pi
0.112
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.72
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cysA-trnT-GGU_p1 TTCTGGAGGGCAGAAACAAC GAACCGATGACTTACGCCTT 50.0 50.0 58.0 58.0 1381-1540 bp 1.0 88.9
cysA-trnT-GGU_p2 CGATTGCAACTACTCCGGAA GAACCGATGACTTACGCCTT 50.0 50.0 58.0 58.0 1205 bp 0.5 59.9
cysA-trnT-GGU_p3 CGATTGCAACTACTCCGGAA GAATCTTTCGAGCAGCTCCA 50.0 50.0 58.0 58.0 1693 bp 0.5 59.8
cysA-trnT-GGU_p4 CGATTGCAACTACTCCGGAA TGAATCTTTCGAGCAGCTCC 50.0 50.0 58.0 58.0 1694 bp 0.5 59.8
cysA-trnT-GGU_p5 CTCGGGGTTTAGCAATTCGA GAACCGATGACTTACGCCTT 50.0 50.0 58.0 58.0 1508 bp 0.5 59.8