Hotspot

trnH-GUG-ycf2

Genome · 30783-31109 · 327 bp

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Pi 0.0767
MarkerSeek score 74.50
Primer available yes
Label trnH-GUG-ycf2

Feature Metadata

label_name
trnH-GUG-ycf2
feature_type
igs
region
Genome
length_bp
327
variable_sites
25
pi
0.0767
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-ycf2_p1 AAGGCAGTGGATTGTGGATC TGTACAAAGATTCCTTTCTTAGTGA 50.0 32.0 57.9 56.2 416-417 bp 1.0 45.0
trnH-GUG-ycf2_p2 AAGGCAGTGGATTGTGGATC ACAAAGATTCCTTTCTTAGTGA 50.0 31.8 57.9 53.0 413-414 bp 1.0 45.0
trnH-GUG-ycf2_p3 GCCAAGTGGATTAAGGCAGT TGTACAAAGATTCCTTTCTTAGTGA 50.0 32.0 58.2 56.2 428-429 bp 1.0 44.9
trnH-GUG-ycf2_p4 AAGGCAGTGGATTGTGGATC CACCCAAATCAAAAATTTTGTACA 50.0 29.2 57.9 55.3 434-435 bp 1.0 44.8
trnH-GUG-ycf2_p5 GCCAAGTGGATTAAGGCAGT CACCCAAATCAAAAATTTTGTACA 50.0 29.2 58.2 55.3 446-447 bp 1.0 44.7