Hotspot

atpI-atpH

Genome · 18303-18726 · 424 bp

Back to Aneura

Pi 0.0963
MarkerSeek score 79.30
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
Genome
length_bp
424
variable_sites
39
pi
0.0963
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 TTCGTGGGGACGATGTTTTT CACACACTCCCTTCCCAAAA 45.0 50.0 58.0 57.9 1520-1551 bp 1.0 85.4
atpI-atpH_p2 TTTCGTGGGGACGATGTTTT CACACACTCCCTTCCCAAAA 45.0 50.0 58.0 57.9 1521-1552 bp 1.0 85.4
atpI-atpH_p3 ACCCTGGCGAGTTTTGAAAT CACACACTCCCTTCCCAAAA 45.0 50.0 58.0 57.9 1495 bp 0.5 59.4
atpI-atpH_p4 ACCCTGGCGAGTTTTGAAAT GCCGGGTCCTATAGAAGCTA 45.0 55.0 58.0 58.1 899 bp 0.5 59.3
atpI-atpH_p5 ACCCTGGCGAGTTTTGAAAT ACGGAAGCAGCAGAAATCAA 45.0 45.0 58.0 58.1 853 bp 0.5 59.1