Hotspot

petN-trnC-GCA

Genome · 5689-6033 · 345 bp

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Pi 0.0849
MarkerSeek score 77.00
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
Genome
length_bp
345
variable_sites
27
pi
0.0849
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 TGCACCCCAAGCTATACTAGT TTCGAATCCGGGTGTCACTT 47.6 50.0 58.2 59.3 372-395 bp 1.0 69.2
petN-trnC-GCA_p2 TGCACCCCAAGCTATACTAGT GTTCGAATCCGGGTGTCAC 47.6 57.9 58.2 58.5 373-396 bp 1.0 67.3
petN-trnC-GCA_p3 TGCACCCCAAGCTATACTAGT TCGAATCCGGGTGTCACTT 47.6 52.6 58.2 58.6 371-394 bp 1.0 66.6
petN-trnC-GCA_p4 TGCACCCCAAGCTATACTAGT TTCGAATCCGGGTGTCACT 47.6 52.6 58.2 58.6 372-395 bp 1.0 66.6
petN-trnC-GCA_p5 TGCACCCCAAGCTATACTAG TTCGAATCCGGGTGTCACTT 50.0 50.0 56.4 59.3 372-395 bp 1.0 66.3