Hotspot

rpoC1

LSC · 21426-24217 · 2792 bp

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Pi 0.00113

MarkerSeek score 52.10

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2792
variable_sites: 6
pi: 0.00113
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	CCGTCCTAGTTGAATCGGAG	TTGCTCGTTCGAGAACTACG	55.0	50.0	57.5	58.0	2978-2983 bp	1.0	76.2
rpoC1_p2	CCGTCCTAGTTGAATCGGAG	ACGATCTTTGGCTCTGGAAC	55.0	50.0	57.5	57.9	2961-2966 bp	1.0	75.7
rpoC1_p3	CCGTCCTAGTTGAATCGGAG	CGATCTTTGGCTCTGGAACT	55.0	50.0	57.5	57.6	2960-2965 bp	1.0	73.3
rpoC1_p4	CCCGTCCTAGTTGAATCGGA	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.9	58.0	2979-2984 bp	1.0	73.0
rpoC1_p5	TCCCGTCCTAGTTGAATCGG	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.9	58.0	2980-2985 bp	1.0	73.0