Hotspot

rpl32-trnL-UAG

SSC · 114830-115711 · 882 bp

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Pi 0.00472

MarkerSeek score 52.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 882
variable_sites: 8
pi: 0.00472
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGGATATTGGGCAGCCTTA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	1006 bp	1.0	79.3
rpl32-trnL-UAG_p2	AGGGATATTGGGCAGCCTTA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	1007 bp	1.0	79.3
rpl32-trnL-UAG_p3	AGGGATATTGGGCAGCCTTA	CTCGCACTGCTTCCTAAGAG	50.0	55.0	58.2	58.1	1027 bp	1.0	78.7
rpl32-trnL-UAG_p4	AGGGATATTGGGCAGCCTTA	CTCTCGCACTGCTTCCTAAG	50.0	55.0	58.2	58.1	1029 bp	1.0	78.7
rpl32-trnL-UAG_p5	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	1016 bp	1.0	78.4