Hotspot

ndhF-rpl32

SSC · 113697-114667 · 971 bp

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Pi 0.00961

MarkerSeek score 55.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 971
variable_sites: 18
pi: 0.00961
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGACGGAAAAAGAAG	TAAGGCTGCCCAATATCCCT	50.0	50.0	58.0	58.2	1156-1210 bp	1.0	82.2
ndhF-rpl32_p2	TGCCGACGGAAAAAGAAGAA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1154-1208 bp	1.0	82.1
ndhF-rpl32_p3	TTGCCGACGGAAAAAGAAGA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1155-1209 bp	1.0	82.1
ndhF-rpl32_p4	GTTGCCGACGGAAAAAGAAG	GACAACGCTTTTAAGGCTGC	50.0	50.0	58.0	58.3	1167-1221 bp	1.0	81.0
ndhF-rpl32_p5	AAAAGAAGAAGTCCCGCTCC	TAAGGCTGCCCAATATCCCT	50.0	50.0	57.8	58.2	1144-1198 bp	1.0	81.0