Hotspot

rpl14-rpl16

LSC · 82055-82201 · 147 bp

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Pi 0.00342

MarkerSeek score 55.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 147
variable_sites: 1
pi: 0.00342
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CAAAAGGGTCTGGGGTTGAA	GGGCGATTTCAATAGCATCG	50.0	50.0	57.9	57.4	222-233 bp	1.0	78.7
rpl14-rpl16_p2	TTCAAAAGGGTCTGGGGTTG	GGGCGATTTCAATAGCATCG	50.0	50.0	57.9	57.4	224-235 bp	1.0	78.7
rpl14-rpl16_p3	ACACATCAATTCTCGAGCCC	GGGCGATTTCAATAGCATCG	50.0	50.0	58.0	57.4	261-272 bp	1.0	78.5
rpl14-rpl16_p4	TCGACGATTGCTAGCTCCTA	GGGCGATTTCAATAGCATCG	50.0	50.0	58.0	57.4	291-302 bp	1.0	78.1
rpl14-rpl16_p5	CCGCTGTTATCCGCTACATT	GGGCGATTTCAATAGCATCG	50.0	50.0	58.1	57.4	242-253 bp	1.0	78.0