Hotspot

psbE-petL

LSC · 65887-66796 · 910 bp

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Pi 0.0022

MarkerSeek score 51.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 910
variable_sites: 4
pi: 0.0022
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.9	58.0	1063-1068 bp	1.0	80.2
psbE-petL_p2	ACTGACGAATAACCAACCCG	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.9	58.0	1076-1081 bp	1.0	79.9
psbE-petL_p3	CAACCCGCAATGAATAGGGA	TGAGGTTACCGTTAAAGCCG	50.0	50.0	57.9	57.9	1066-1071 bp	1.0	79.6
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.8	58.0	986-991 bp	1.0	79.4
psbE-petL_p5	ACTGACGAATAACCAACCCG	TGAGGTTACCGTTAAAGCCG	50.0	50.0	57.9	57.9	1079-1084 bp	1.0	79.3