Hotspot

trnT-GGU-psbD

LSC · 32593-33824 · 1232 bp

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Pi 0.00325

MarkerSeek score 52.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1232
variable_sites: 8
pi: 0.00325
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AAGGGCCCTTTTAACTCAGC	AGACCGGACCATCCTACAAA	50.0	50.0	58.1	58.1	1413-1425 bp	1.0	79.7
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	AGACCGGACCATCCTACAAA	50.0	50.0	57.9	58.1	1409-1421 bp	1.0	79.5
trnT-GGU-psbD_p3	AAGGGCCCTTTTAACTCAGC	GAGCAATAGACCGGACCATC	50.0	55.0	58.1	57.8	1420-1432 bp	1.0	78.9
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	GAGCAATAGACCGGACCATC	50.0	55.0	57.9	57.8	1416-1428 bp	1.0	78.7
trnT-GGU-psbD_p5	AAGGGCCCTTTTAACTCAGC	AGAGCAATAGACCGGACCAT	50.0	50.0	58.1	58.2	1421-1433 bp	1.0	78.5