Hotspot

trnE-UUC-trnT-GGU

LSC · 31850-32520 · 671 bp

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Pi 0.00571
MarkerSeek score 57.90
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
671
variable_sites
7
pi
0.00571
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 748-797 bp 1.0 82.1
trnE-UUC-trnT-GGU_p2 GTGTAGTACCCTACCCCCAG GAACCGATGACTTACGCCTT 60.0 50.0 58.2 58.0 783-832 bp 1.0 81.6
trnE-UUC-trnT-GGU_p3 CGTTGCCTCCTTGAAAGAGA TACCGCTGAGTTAAAAGGGC 50.0 50.0 57.8 57.9 713-762 bp 1.0 81.5
trnE-UUC-trnT-GGU_p4 GTGTAGTACCCTACCCCCAG TACCGCTGAGTTAAAAGGGC 60.0 50.0 58.2 57.9 748-797 bp 1.0 81.0
trnE-UUC-trnT-GGU_p5 CGTTGCCTCCTTGAAAGAGA CCGATGACTTACGCCTTACC 50.0 55.0 57.8 58.2 745-794 bp 1.0 81.0