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trnK-UUU-rps16

LSC · 4521-5266 · 746 bp

Back to Anethum

Pi 0.00694

MarkerSeek score 59.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 10
pi: 0.00694
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCCCTCGCTTCATCCAAATG	AGGAACTGTTCGGGATCTTT	50.0	45.0	58.0	56.5	868-875 bp	1.0	68.9
trnK-UUU-rps16_p2	CCGATGGTATGGACGAATCC	AGGAACTGTTCGGGATCTTT	55.0	45.0	57.9	56.5	885-892 bp	1.0	68.6
trnK-UUU-rps16_p3	CGATGGTATGGACGAATCCC	AGGAACTGTTCGGGATCTTT	55.0	45.0	57.9	56.5	884-891 bp	1.0	68.6
trnK-UUU-rps16_p4	AAAGCCGAGTACTCTACCGT	AGGAACTGTTCGGGATCTTT	50.0	45.0	58.2	56.5	824-831 bp	1.0	68.0
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	AGGAACTGTTCGGGATCTTT	50.0	45.0	58.2	56.5	823-830 bp	1.0	68.0