Hotspot

trnH-GUG-psbA

LSC · 468-658 · 191 bp

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Pi 0.0131

MarkerSeek score 53.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 191
variable_sites: 5
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TTTACTTGGTTGGCAGGGTC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.0	57.9	336-347 bp	1.0	83.3
trnH-GUG-psbA_p2	TTTACTTGGTTGGCAGGGTC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.0	57.9	339-350 bp	1.0	83.2
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	297-308 bp	1.0	83.2
trnH-GUG-psbA_p4	TTTACTTGGTTGGCAGGGTC	GCGCTAACCTTGGTATGGAA	50.0	50.0	58.0	58.0	386-397 bp	1.0	83.1
trnH-GUG-psbA_p5	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	311-322 bp	1.0	82.9