Hotspot

rps2

LSC · 15811-16524 · 714 bp

Back to Anerincleistus

Pi 0.0115

MarkerSeek score 49.40

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 714
variable_sites: 19
pi: 0.0115
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	GGCTCACGTCTCGAATGAAT	TCTGTTACCGCGCTATCCTA	50.0	50.0	58.1	58.0	1807-1832 bp	1.0	81.8
rps2_p2	GGCTCACGTCTCGAATGAAT	CGAGTTTTAGCCAAAGCAGC	50.0	50.0	58.1	58.0	1718-1743 bp	1.0	81.8
rps2_p3	TTCTCCAATCTGGGTTTGGC	TCTGTTACCGCGCTATCCTA	50.0	50.0	58.1	58.0	1824-1849 bp	0.714286	68.6
rps2_p4	GGCTCACGTCTCGAATGAAT	TTCCGTCGGAACAATTGGTT	50.0	45.0	58.1	58.0	1256-1259 bp	0.714286	68.3
rps2_p5	TTCTCCAATCTGGGTTTGGC	TTCCGTCGGAACAATTGGTT	50.0	45.0	58.1	58.0	1273-1276 bp	0.714286	68.3