Hotspot

rps16

LSC · 4695-5757 · 1063 bp

Back to Anerincleistus

Pi 0.00947

MarkerSeek score 53.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1063
variable_sites: 25
pi: 0.00947
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CCTTTCAGGATCAGTCGTGG	ATTTAACCCGCTCAGTCAGC	55.0	50.0	58.0	58.3	2019-2095 bp	1.0	81.9
rps16_p2	TCAGGATCAGTCGTGGTCTT	ATTTAACCCGCTCAGTCAGC	50.0	50.0	58.1	58.3	2015-2091 bp	1.0	81.4
rps16_p3	TTCAGGATCAGTCGTGGTCT	ATTTAACCCGCTCAGTCAGC	50.0	50.0	58.1	58.3	2016-2092 bp	1.0	81.4
rps16_p4	CGACCCCCATTCTGATTCTG	ATTTAACCCGCTCAGTCAGC	55.0	50.0	58.0	58.3	2179 bp	0.142857	43.3
rps16_p5	TCGACCCCCATTCTGATTCT	ATTTAACCCGCTCAGTCAGC	50.0	50.0	58.1	58.3	2180 bp	0.142857	42.7