Hotspot

ndhD-psaC

SSC · 119665-119806 · 142 bp

Back to Anerincleistus

Pi 0.0314

MarkerSeek score 70.10

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 142
variable_sites: 9
pi: 0.0314
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	ACCCGCTAATATTGGTAAACCT	AAACAACTCGAAGCATGGGT	40.9	45.0	57.2	58.0	228-234 bp	1.0	61.7
ndhD-psaC_p2	ACCCGCTAATATTGGTAAACCT	GGCATGAAACAACTCGAAGC	40.9	50.0	57.2	58.0	234-240 bp	1.0	61.5
ndhD-psaC_p3	AACCCGCTAATATTGGTAAACCT	AAACAACTCGAAGCATGGGT	39.1	45.0	57.9	58.0	229-235 bp	1.0	59.0
ndhD-psaC_p4	AACCCGCTAATATTGGTAAACCT	GGCATGAAACAACTCGAAGC	39.1	50.0	57.9	58.0	235-241 bp	1.0	58.8
ndhD-psaC_p5	ACCCGCTAATATTGGTAAACCT	AGAGATGTGAATCCGCTTGC	40.9	50.0	57.2	58.3	288-294 bp	1.0	58.3