Hotspot

ndhF-rpl32

SSC · 115289-116274 · 986 bp

Back to Anerincleistus

Pi 0.025
MarkerSeek score 72.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
986
variable_sites
60
pi
0.025
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGGGAAGTGGAATGAGAGGT CTTCAATGCTGTAAAATAGGCT 50.0 36.4 58.0 54.8 612-1217 bp 1.0 48.5
ndhF-rpl32_p2 TGGGAAGTGGAATGAGAGGT CTTCAATGCTGTAAAATAGGCTT 50.0 34.8 58.0 55.5 612-1217 bp 1.0 48.5
ndhF-rpl32_p3 TGGGAAGTGGAATGAGAGGT TCAATGCTGTAAAATAGGCT 50.0 35.0 58.0 52.5 610-1215 bp 1.0 48.5
ndhF-rpl32_p4 TGGGAAGTGGAATGAGAGGT TCAATGCTGTAAAATAGGCTT 50.0 33.3 58.0 53.5 610-1215 bp 1.0 48.5
ndhF-rpl32_p5 TGGGAAGTGGAATGAGAGGT TTCAATGCTGTAAAATAGGCT 50.0 33.3 58.0 53.5 611-1216 bp 1.0 48.5