Hotspot

ycf2-trnL-CAA

IRb · 95449-96083 · 635 bp

Back to Anerincleistus

Pi 0.00615

MarkerSeek score 61.60

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 635
variable_sites: 9
pi: 0.00615
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	GGTTGTTGCTATCTGCTCCA	AAATGGTAGACACGCGAGAC	50.0	50.0	57.9	58.0	742-744 bp	1.0	80.2
ycf2-trnL-CAA_p2	GGTTGTTGCTATCTGCTCCA	TGAAATGGTAGACACGCGAG	50.0	50.0	57.9	58.0	744-746 bp	1.0	80.2
ycf2-trnL-CAA_p3	AACGGTTGTTGCTATCTGCT	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	745-747 bp	1.0	79.6
ycf2-trnL-CAA_p4	AACGGTTGTTGCTATCTGCT	TGAAATGGTAGACACGCGAG	45.0	50.0	57.8	58.0	747-749 bp	1.0	79.6
ycf2-trnL-CAA_p5	GGTTGTTGCTATCTGCTCCA	GCCTTGGTGGTGAAATGGTA	50.0	50.0	57.9	58.1	754-756 bp	1.0	79.6