Hotspot

psbZ-trnG-UCC

LSC · 37285-37580 · 296 bp

Back to Anerincleistus

Pi 0.028

MarkerSeek score 69.80

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 296
variable_sites: 19
pi: 0.028
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	GACGGCTGGTCAAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.1	57.8	442-502 bp	1.0	82.8
psbZ-trnG-UCC_p2	CCCGTTGTTTTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.9	57.8	463-523 bp	1.0	82.5
psbZ-trnG-UCC_p3	ACGGCTGGTCAAGTAACAAA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.7	57.8	441-501 bp	1.0	81.2
psbZ-trnG-UCC_p4	GTTTTTGCTTCTCCTGACGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.3	57.8	457-517 bp	1.0	78.2
psbZ-trnG-UCC_p5	GACGGCTGGTCAAGTAACAA	AACTTAACTCGGGCGGATAG	50.0	50.0	58.1	57.1	477-537 bp	1.0	76.7