Hotspot

trnS-UGA-psbZ

LSC · 36719-37095 · 377 bp

Back to Anerincleistus

Pi 0.0225

MarkerSeek score 62.10

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 377
variable_sites: 18
pi: 0.0225
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	TTTTCAAGACCGGAGCCATC	TTGTTACTTGACCAGCCGTC	50.0	50.0	58.2	58.1	527 bp	1.0	80.3
trnS-UGA-psbZ_p2	TTTTCAAGACCGGAGCCATC	GGAGAAGCAAAAACAACGGG	50.0	50.0	58.2	57.9	506 bp	1.0	80.3
trnS-UGA-psbZ_p3	TTTTCAAGACCGGAGCCATC	TTTGTTACTTGACCAGCCGT	50.0	45.0	58.2	57.7	528 bp	1.0	78.6
trnS-UGA-psbZ_p4	AGCAAAAGGAGAGAGAGGGA	TTGTTACTTGACCAGCCGTC	50.0	50.0	57.7	58.1	586 bp	0.857143	73.1
trnS-UGA-psbZ_p5	AGCAAAAGGAGAGAGAGGGA	GGAGAAGCAAAAACAACGGG	50.0	50.0	57.7	57.9	565 bp	0.857143	73.1