Hotspot

petN-psbM

LSC · 29684-30034 · 351 bp

Back to Anerincleistus

Pi 0.013

MarkerSeek score 67.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 351
variable_sites: 12
pi: 0.013
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTCT	45.0	42.9	58.0	57.0	483-511 bp	1.0	69.8
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTCT	50.0	42.9	57.8	57.0	489-517 bp	1.0	68.2
petN-psbM_p3	GGCTGCTTTAATGGTCGTCT	TGCTACTGCACTCTTCATTCT	50.0	42.9	58.3	57.0	474-502 bp	1.0	67.8
petN-psbM_p4	TCTGTCCCTCGTAGTATGGG	TGCTACTGCACTCTTCATTCT	55.0	42.9	57.6	57.0	444-472 bp	1.0	67.4
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTC	45.0	45.0	58.0	55.5	483-511 bp	1.0	65.4