Hotspot

trnH-GUG-psbA

LSC · 76-329 · 254 bp

Back to Anerincleistus

Pi 0.059

MarkerSeek score 72.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 254
variable_sites: 35
pi: 0.059
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TGTTGAAGTTCCATCTACAAATGG	55.0	37.5	58.1	57.4	279-304 bp	1.0	54.4
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TGTTGAAGTTCCATCTACAAATGG	50.0	37.5	57.9	57.4	293-318 bp	1.0	54.3
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TGTTGAAGTTCCATCTACAAATGG	50.0	37.5	57.9	57.4	292-317 bp	1.0	54.3
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TGTTGAAGTTCCATCTACAAATGG	50.0	37.5	57.9	57.4	294-319 bp	1.0	54.3
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	TGTTGAAGTTCCATCTACAAATGG	50.0	37.5	57.5	57.4	308-333 bp	1.0	52.0