Hotspot

rpoA

IRa · 165133-166176 · 1044 bp

Back to Anemopaegma

Pi 0.0234
MarkerSeek score 58.40
Primer available yes
Label rpoA

Feature Metadata

label_name
rpoA
feature_type
gene
region
IRa
length_bp
1044
variable_sites
82
pi
0.0234
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoA_p1 AAAGTCTGAGACGACCCAGA AGGGTACGAGAAAAGGGACA 50.0 50.0 58.0 58.0 2232-2408 bp 1.0 88.1
rpoA_p2 AAGTCTGAGACGACCCAGAA AGGGTACGAGAAAAGGGACA 50.0 50.0 58.0 58.0 2231-2407 bp 1.0 88.1
rpoA_p3 AGTCTGAGACGACCCAGAAA AGGGTACGAGAAAAGGGACA 50.0 50.0 58.0 58.0 2230-2406 bp 1.0 88.1
rpoA_p4 AGTCTGAGACGACCCAGAAA CCGAGAACTGGTTCACGTAG 50.0 55.0 58.0 58.0 2471-2647 bp 1.0 88.0
rpoA_p5 AGTCTGAGACGACCCAGAAA CGAGAACTGGTTCACGTAGG 50.0 55.0 58.0 58.0 2470-2646 bp 1.0 88.0
rpoA_p1 AGGGTACGAGAAAAGGGACA AAAGTCTGAGACGACCCAGA 50.0 50.0 58.0 58.0 2232-2408 bp 1.0 87.8
rpoA_p2 AGGGTACGAGAAAAGGGACA AAGTCTGAGACGACCCAGAA 50.0 50.0 58.0 58.0 2231-2407 bp 1.0 87.8
rpoA_p3 AGGGTACGAGAAAAGGGACA AGTCTGAGACGACCCAGAAA 50.0 50.0 58.0 58.0 2230-2406 bp 1.0 87.8
rpoA_p4 CGAGAACTGGTTCACGTAGG AAGTCTGAGACGACCCAGAA 55.0 50.0 58.0 58.0 2471-2647 bp 1.0 87.7
rpoA_p5 CGAGAACTGGTTCACGTAGG AGTCTGAGACGACCCAGAAA 55.0 50.0 58.0 58.0 2470-2646 bp 1.0 87.7