Hotspot

rps11-rpoA

IRa · 165023-165132 · 110 bp

Back to Anemopaegma

Pi 0.0835

MarkerSeek score 64.70

Primer available yes

Label rps11-rpoA

Feature Metadata

label_name: rps11-rpoA
feature_type: igs
region: IRa
length_bp: 110
variable_sites: 26
pi: 0.0835
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpoA_p1	AGGGTACGAGAAAAGGGACA	CGCGTTGCAATGCCTATTAG	50.0	50.0	58.0	58.0	466-579 bp	1.0	90.4
rps11-rpoA_p2	CCGAGAACTGGTTCACGTAG	CGCGTTGCAATGCCTATTAG	55.0	50.0	58.0	58.0	707-820 bp	1.0	89.1
rps11-rpoA_p3	CGAGAACTGGTTCACGTAGG	CGCGTTGCAATGCCTATTAG	55.0	50.0	58.0	58.0	706-819 bp	1.0	89.1
rps11-rpoA_p4	CGAGAACTGGTTCACGTAGG	TACCAAGTGCTTCTACCGGA	55.0	50.0	58.0	58.1	1227-1340 bp	1.0	88.0
rps11-rpoA_p5	AGGGTACGAGAAAAGGGACA	TACCAAGTGCTTCTACCGGA	50.0	50.0	58.0	58.1	987-1100 bp	1.0	87.7