Hotspot

ycf1-trnN-GUU

IRa · 135381-136247 · 867 bp

Back to Anemopaegma

Pi 0.05

MarkerSeek score 77.50

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 867
variable_sites: 88
pi: 0.05
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	ACGACAACGACCGAATTGAT	AAATCGTGATCGGGATAGCG	45.0	50.0	57.9	57.9	919-1467 bp	0.875	82.3
ycf1-trnN-GUU_p2	GCCCTATGGAAAATGCGGTA	AAATCGTGATCGGGATAGCG	50.0	50.0	58.0	57.9	1033-1508 bp	0.5	64.5
ycf1-trnN-GUU_p3	GCCCTATGGAAAATGCGGTA	GTGTGGGTCTTCAGCGTATT	50.0	50.0	58.0	57.9	991-1466 bp	0.5	64.2
ycf1-trnN-GUU_p4	GCCCTATGGAAAATGCGGTA	TTAACGGTCAAAGTGAGCCC	50.0	50.0	58.0	58.1	646-1121 bp	0.5	64.1
ycf1-trnN-GUU_p5	GCCCTATGGAAAATGCGGTA	GGTAAACGCGCTTTCACATC	50.0	50.0	58.0	58.1	1253-1722 bp	0.5	64.1