Hotspot

rps15-ndhF

SSC · 115740-115760 · 21 bp

Back to Anemopaegma

Pi 0.0714

MarkerSeek score 66.90

Primer available yes

Label rps15-ndhF

Feature Metadata

label_name: rps15-ndhF
feature_type: igs
region: SSC
length_bp: 21
variable_sites: 4
pi: 0.0714
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhF_p1	TTGGGAAAACGTCAACGACT	CAAATAGGGGACGGCCTTTT	45.0	50.0	58.0	58.2	757-794 bp	1.0	84.5
rps15-ndhF_p2	TTGGGAAAACGTCAACGACT	CTGGGGCGATAAGAGGATTG	45.0	55.0	58.0	57.8	286-323 bp	1.0	83.7
rps15-ndhF_p3	TTGGGAAAACGTCAACGACT	ACTGGGGCGATAAGAGGATT	45.0	50.0	58.0	58.2	287-324 bp	1.0	83.5
rps15-ndhF_p4	TTGGGAAAACGTCAACGACT	CTTGACTGGGGCGATAAGAG	45.0	55.0	58.0	57.8	291-328 bp	1.0	83.2
rps15-ndhF_p5	TTGGGAAAACGTCAACGACT	AAAACAGGCCCGAAGAGAAG	45.0	50.0	58.0	58.1	389-426 bp	0.875	79.1