Hotspot

rpoA-rps11

IRb · 79077-79186 · 110 bp

Back to Anemopaegma

Pi 0.116

MarkerSeek score 69.10

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: IRb
length_bp: 110
variable_sites: 35
pi: 0.116
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	CGCGTTGCAATGCCTATTAG	AGGGTACGAGAAAAGGGACA	50.0	50.0	58.0	58.0	466-579 bp	1.0	91.7
rpoA-rps11_p2	CGCGTTGCAATGCCTATTAG	CCGAGAACTGGTTCACGTAG	50.0	55.0	58.0	58.0	707-820 bp	1.0	90.1
rpoA-rps11_p3	CGCGTTGCAATGCCTATTAG	CGAGAACTGGTTCACGTAGG	50.0	55.0	58.0	58.0	706-819 bp	1.0	90.1
rpoA-rps11_p4	TACCAAGTGCTTCTACCGGA	CGAGAACTGGTTCACGTAGG	50.0	55.0	58.1	58.0	1227-1340 bp	1.0	88.5
rpoA-rps11_p5	TACCAAGTGCTTCTACCGGA	AGGGTACGAGAAAAGGGACA	50.0	50.0	58.1	58.0	987-1100 bp	1.0	88.1