Hotspot

trnS-GCU-trnG-UCC

LSC · 8756-9474 · 719 bp

Back to Anemopaegma

Pi 0.00834
MarkerSeek score 62.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
719
variable_sites
18
pi
0.00834
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 781-800 bp 1.0 54.8
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 780-799 bp 1.0 54.8
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 784-803 bp 1.0 53.8
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 783-802 bp 1.0 53.8
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 782-801 bp 1.0 53.8