Hotspot

ndhE-ndhG

SSC · 119855-120114 · 260 bp

Back to Anemone

Pi 0.087

MarkerSeek score 75.80

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 260
variable_sites: 65
pi: 0.087
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TCAAGAAATCGGTTGGGCAA	TGGACTGTTGGAGATGGAGT	45.0	50.0	58.0	58.0	1234-1271 bp	1.0	89.4
ndhE-ndhG_p2	TCAAGAAATCGGTTGGGCAA	CCATTTTGTAGCTGCTGCAC	45.0	50.0	58.0	58.0	1346-1383 bp	1.0	89.1
ndhE-ndhG_p3	CGTCCCAGGGTATCATCTCT	TGGACTGTTGGAGATGGAGT	55.0	50.0	58.0	58.0	1141-1178 bp	0.818182	80.5
ndhE-ndhG_p4	CGTCCCAGGGTATCATCTCT	CCATTTTGTAGCTGCTGCAC	55.0	50.0	58.0	58.0	1253-1290 bp	0.818182	80.3
ndhE-ndhG_p5	CGTCCCAGGGTATCATCTCT	GTGGGAAGTCTAGGAGTGGT	55.0	55.0	58.0	58.1	1357-1394 bp	0.818182	79.5