Hotspot

ndhC-trnV-UAC

LSC · 51457-52585 · 1129 bp

Back to Anemone

Pi 0.0473
MarkerSeek score 84.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1129
variable_sites
164
pi
0.0473
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.97
markerseek_score
84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 36.4 50.0 56.1 57.8 1092-1213 bp 1.0 59.3
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGAAACA GAAGGTCTACGGTTCGAGTC 36.4 55.0 56.1 57.5 1091-1212 bp 1.0 56.6
ndhC-trnV-UAC_p3 TCATATTCGTGAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 36.4 55.0 56.1 58.7 1080-1201 bp 1.0 55.4
ndhC-trnV-UAC_p4 CATATTCGTGAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 38.1 50.0 54.6 57.8 1091-1212 bp 1.0 55.3
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGAAACAT AGAAGGTCTACGGTTCGAGT 34.8 50.0 56.6 57.8 1092-1213 bp 1.0 55.3