Hotspot

trnS-GCU-trnL-UAA

LSC · 47695-48394 · 700 bp

Back to Anemone

Pi 0.0325

MarkerSeek score 76.30

Primer available yes

Label trnS-GCU-trnL-UAA

Feature Metadata

label_name: trnS-GCU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 700
variable_sites: 76
pi: 0.0325
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnL-UAA_p1	CAATCCGACGCTTTAGTCCA	CTACCAATTCCGCCATACCC	50.0	55.0	58.0	58.1	685-825 bp	1.0	91.1
trnS-GCU-trnL-UAA_p2	AATCCGACGCTTTAGTCCAC	CTACCAATTCCGCCATACCC	50.0	55.0	58.0	58.1	684-824 bp	1.0	91.1
trnS-GCU-trnL-UAA_p3	CAATCCGACGCTTTAGTCCA	AATCCAACCAAGTCCGTAGC	50.0	50.0	58.0	57.9	707-847 bp	1.0	91.0
trnS-GCU-trnL-UAA_p4	AATCCGACGCTTTAGTCCAC	AATCCAACCAAGTCCGTAGC	50.0	50.0	58.0	57.9	706-846 bp	1.0	91.0
trnS-GCU-trnL-UAA_p5	CAATCCGACGCTTTAGTCCA	CCGTAGCGTCTACCAATTCC	50.0	55.0	58.0	58.2	694-834 bp	1.0	90.8