Hotspot

trnD-GUC-psbM

LSC · 24690-25767 · 1078 bp

Back to Anemone

Pi 0.0283

MarkerSeek score 78.20

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1078
variable_sites: 104
pi: 0.0283
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	GTTCGAGCCCCGTCAGTC	AGAATGAACAGTGCAGTAGC	66.7	45.0	60.1	55.8	869-1204 bp	1.0	51.3
trnD-GUC-psbM_p2	GTTCGAGCCCCGTCAGTC	TCGTTTTGACTGACCGTTTT	66.7	40.0	60.1	56.1	923-1258 bp	1.0	51.0
trnD-GUC-psbM_p3	GTTCGAGCCCCGTCAGTC	AATCGTTTTGACTGACCGTT	66.7	40.0	60.1	55.9	925-1260 bp	1.0	51.0
trnD-GUC-psbM_p4	GTTCGAGCCCCGTCAGTC	ATCGTTTTGACTGACCGTTT	66.7	40.0	60.1	55.9	924-1259 bp	1.0	51.0
trnD-GUC-psbM_p5	GTTCGAGCCCCGTCAGTC	TCGTTTTGACTGACCGTTTTT	66.7	38.1	60.1	56.9	923-1258 bp	1.0	51.0