Hotspot

trnT-GGU-trnE-UUC

LSC · 23307-23991 · 685 bp

Back to Anemone

Pi 0.0197
MarkerSeek score 76.30
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
685
variable_sites
49
pi
0.0197
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 GAACCGATGACTTACGCCTT AGCCTCCTTTCCTAGTACCC 50.0 55.0 58.0 57.8 814-874 bp 1.0 87.2
trnT-GGU-trnE-UUC_p2 GAACCGATGACTTACGCCTT GCCTCCTTTCCTAGTACCCT 50.0 55.0 58.0 57.8 813-873 bp 1.0 87.2
trnT-GGU-trnE-UUC_p3 GAACCGATGACTTACGCCTT GCTGCCTCCTTGAAAGAGAG 50.0 55.0 58.0 58.3 769-829 bp 1.0 86.8
trnT-GGU-trnE-UUC_p4 TACCGCTGAGTTAAAAGGGC AGCCTCCTTTCCTAGTACCC 50.0 55.0 57.9 57.8 779-839 bp 1.0 86.6
trnT-GGU-trnE-UUC_p5 TACCGCTGAGTTAAAAGGGC GCCTCCTTTCCTAGTACCCT 50.0 55.0 57.9 57.8 778-838 bp 1.0 86.6