Hotspot

trnS-GGA-ycf3

LSC · 8981-9854 · 874 bp

Back to Anemone

Pi 0.0244

MarkerSeek score 76.30

Primer available yes

Label trnS-GGA-ycf3

Feature Metadata

label_name: trnS-GGA-ycf3
feature_type: igs
region: LSC
length_bp: 874
variable_sites: 66
pi: 0.0244
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-ycf3_p1	CCCTCGGTAAACAAAAGCCT	CATTTATCCGCGATCTAGGCA	50.0	47.6	58.1	58.0	912-963 bp	1.0	80.6
trnS-GGA-ycf3_p2	ACGGAAAGAGAGGGATTCGA	CATTTATCCGCGATCTAGGCA	50.0	47.6	58.2	58.0	933-984 bp	1.0	80.0
trnS-GGA-ycf3_p3	TTCCAATGCTACGCCTTGAA	CATTTATCCGCGATCTAGGCA	45.0	47.6	57.8	58.0	883-934 bp	1.0	80.0
trnS-GGA-ycf3_p4	ACCCTCGGTAAACAAAAGCC	CATTTATCCGCGATCTAGGCA	50.0	47.6	58.4	58.0	913-964 bp	1.0	78.2
trnS-GGA-ycf3_p5	CCCTCGGTAAACAAAAGCCT	TTTATCCGCGATCTAGGCAT	50.0	45.0	58.1	56.6	910-961 bp	1.0	77.6