Hotspot

rps16-trnK-UUU

LSC · 2355-3179 · 825 bp

Back to Anemone

Pi 0.0662
MarkerSeek score 84.30
Primer available yes
Label rps16-trnK-UUU

Feature Metadata

label_name
rps16-trnK-UUU
feature_type
igs
region
LSC
length_bp
825
variable_sites
151
pi
0.0662
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.95
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnK-UUU_p1 CATTAATTACGCGAAATCAAAT AAAGCCGAGTACTCTACCGT 27.3 50.0 52.0 58.2 791-963 bp 0.818182 44.7
rps16-trnK-UUU_p2 CATTAATTACGCGAAATCAAAT AAGCCGAGTACTCTACCGTT 27.3 50.0 52.0 58.2 790-962 bp 0.818182 44.7
rps16-trnK-UUU_p3 CATTAATTACGCGAAATCAAATT AAAGCCGAGTACTCTACCGT 26.1 50.0 52.9 58.2 791-963 bp 0.818182 44.7
rps16-trnK-UUU_p4 CATTAATTACGCGAAATCAAATT AAGCCGAGTACTCTACCGTT 26.1 50.0 52.9 58.2 790-962 bp 0.818182 44.7
rps16-trnK-UUU_p5 CATTAATTACGCGAAATCAAAT TACCGTTGAGTTAGCAACCC 27.3 50.0 52.0 57.5 777-949 bp 0.818182 44.7