Hotspot

rps4-rps16

LSC · 631-1215 · 585 bp

Back to Anemone

Pi 0.0504

MarkerSeek score 86.70

Primer available yes

Label rps4-rps16

Feature Metadata

label_name: rps4-rps16
feature_type: igs
region: LSC
length_bp: 585
variable_sites: 93
pi: 0.0504
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-rps16_p1	GTAAGTGGGTCGGTTTGGAA	AAGTCGCACGTTGCTTTCTA	50.0	45.0	57.7	58.1	608-989 bp	1.0	88.8
rps4-rps16_p2	AAGTGGGTCGGTTTGGAAAT	AAGTCGCACGTTGCTTTCTA	45.0	45.0	57.6	58.1	606-987 bp	1.0	87.9
rps4-rps16_p3	AGTAAGTGGGTCGGTTTGGA	AAGTCGCACGTTGCTTTCTA	50.0	45.0	58.6	58.1	609-990 bp	1.0	86.3
rps4-rps16_p4	GTAAGTGGGTCGGTTTGGAA	CGTTGCTTTCTACCACATCG	50.0	50.0	57.7	57.2	600-981 bp	1.0	83.6
rps4-rps16_p5	GTAAGTGGGTCGGTTTGGAA	AGTCGCACGTTGCTTTCTAC	50.0	50.0	57.7	58.9	607-988 bp	1.0	83.0