primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps4-rps16_p1	rps4-rps16	1	GTAAGTGGGTCGGTTTGGAA	AAGTCGCACGTTGCTTTCTA	20	20	50.0	45.0	57.7	58.1	0.0	8.1	0.398590	631	1215	608-989	1.000000	88.8
rps4-rps16_p2	rps4-rps16	2	AAGTGGGTCGGTTTGGAAAT	AAGTCGCACGTTGCTTTCTA	20	20	45.0	45.0	57.6	58.1	0.0	8.1	0.514338	631	1215	606-987	1.000000	87.9
rps4-rps16_p3	rps4-rps16	3	AGTAAGTGGGTCGGTTTGGA	AAGTCGCACGTTGCTTTCTA	20	20	50.0	45.0	58.6	58.1	0.0	8.1	0.712574	631	1215	609-990	1.000000	86.3
rps4-rps16_p4	rps4-rps16	4	GTAAGTGGGTCGGTTTGGAA	CGTTGCTTTCTACCACATCG	20	20	50.0	50.0	57.7	57.2	0.0	0.0	1.046989	631	1215	600-981	1.000000	83.6
rps4-rps16_p5	rps4-rps16	5	GTAAGTGGGTCGGTTTGGAA	AGTCGCACGTTGCTTTCTAC	20	20	50.0	50.0	57.7	58.9	0.0	8.1	1.121753	631	1215	607-988	1.000000	83.0
rps16-trnK-UUU_p1	rps16-trnK-UUU	1	CATTAATTACGCGAAATCAAAT	AAAGCCGAGTACTCTACCGT	22	20	27.3	50.0	52.0	58.2	15.9	20.3	8.158880	2355	3179	791-963	0.818182	44.7
rps16-trnK-UUU_p2	rps16-trnK-UUU	2	CATTAATTACGCGAAATCAAAT	AAGCCGAGTACTCTACCGTT	22	20	27.3	50.0	52.0	58.2	15.9	20.3	8.158880	2355	3179	790-962	0.818182	44.7
rps16-trnK-UUU_p3	rps16-trnK-UUU	3	CATTAATTACGCGAAATCAAATT	AAAGCCGAGTACTCTACCGT	23	20	26.1	50.0	52.9	58.2	15.9	20.3	8.301402	2355	3179	791-963	0.818182	44.7
rps16-trnK-UUU_p4	rps16-trnK-UUU	4	CATTAATTACGCGAAATCAAATT	AAGCCGAGTACTCTACCGTT	23	20	26.1	50.0	52.9	58.2	15.9	20.3	8.301402	2355	3179	790-962	0.818182	44.7
rps16-trnK-UUU_p5	rps16-trnK-UUU	5	CATTAATTACGCGAAATCAAAT	TACCGTTGAGTTAGCAACCC	22	20	27.3	50.0	52.0	57.5	15.9	0.0	8.451218	2355	3179	777-949	0.818182	44.7
trnS-GGA-ycf3_p1	trnS-GGA-ycf3	1	CCCTCGGTAAACAAAAGCCT	CATTTATCCGCGATCTAGGCA	20	21	50.0	47.6	58.1	58.0	0.0	8.2	1.134847	8981	9854	912-963	1.000000	80.6
trnS-GGA-ycf3_p2	trnS-GGA-ycf3	2	ACGGAAAGAGAGGGATTCGA	CATTTATCCGCGATCTAGGCA	20	21	50.0	47.6	58.2	58.0	0.0	8.2	1.191833	8981	9854	933-984	1.000000	80.0
trnS-GGA-ycf3_p3	trnS-GGA-ycf3	3	TTCCAATGCTACGCCTTGAA	CATTTATCCGCGATCTAGGCA	20	21	45.0	47.6	57.8	58.0	5.6	8.2	1.233148	8981	9854	883-934	1.000000	80.0
trnS-GGA-ycf3_p4	trnS-GGA-ycf3	4	ACCCTCGGTAAACAAAAGCC	CATTTATCCGCGATCTAGGCA	20	21	50.0	47.6	58.4	58.0	0.0	8.2	1.427808	8981	9854	913-964	1.000000	78.2
trnS-GGA-ycf3_p5	trnS-GGA-ycf3	5	CCCTCGGTAAACAAAAGCCT	TTTATCCGCGATCTAGGCAT	20	20	50.0	45.0	58.1	56.6	0.0	8.2	1.514239	8981	9854	910-961	1.000000	77.6
trnT-GGU-trnE-UUC_p1	trnT-GGU-trnE-UUC	1	GAACCGATGACTTACGCCTT	AGCCTCCTTTCCTAGTACCC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.183782	23307	23991	814-874	1.000000	87.2
trnT-GGU-trnE-UUC_p2	trnT-GGU-trnE-UUC	2	GAACCGATGACTTACGCCTT	GCCTCCTTTCCTAGTACCCT	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.183782	23307	23991	813-873	1.000000	87.2
trnT-GGU-trnE-UUC_p3	trnT-GGU-trnE-UUC	3	GAACCGATGACTTACGCCTT	GCTGCCTCCTTGAAAGAGAG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.269359	23307	23991	769-829	1.000000	86.8
trnT-GGU-trnE-UUC_p4	trnT-GGU-trnE-UUC	4	TACCGCTGAGTTAAAAGGGC	AGCCTCCTTTCCTAGTACCC	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.281349	23307	23991	779-839	1.000000	86.6
trnT-GGU-trnE-UUC_p5	trnT-GGU-trnE-UUC	5	TACCGCTGAGTTAAAAGGGC	GCCTCCTTTCCTAGTACCCT	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.281349	23307	23991	778-838	1.000000	86.6
trnD-GUC-psbM_p1	trnD-GUC-psbM	1	GTTCGAGCCCCGTCAGTC	AGAATGAACAGTGCAGTAGC	18	20	66.7	45.0	60.1	55.8	0.0	0.0	6.355329	24690	25767	869-1204	1.000000	51.3
trnD-GUC-psbM_p2	trnD-GUC-psbM	2	GTTCGAGCCCCGTCAGTC	TCGTTTTGACTGACCGTTTT	18	20	66.7	40.0	60.1	56.1	0.0	0.0	5.983440	24690	25767	923-1258	1.000000	51.0
trnD-GUC-psbM_p3	trnD-GUC-psbM	3	GTTCGAGCCCCGTCAGTC	AATCGTTTTGACTGACCGTT	18	20	66.7	40.0	60.1	55.9	0.0	0.0	6.212214	24690	25767	925-1260	1.000000	51.0
trnD-GUC-psbM_p4	trnD-GUC-psbM	4	GTTCGAGCCCCGTCAGTC	ATCGTTTTGACTGACCGTTT	18	20	66.7	40.0	60.1	55.9	0.0	0.0	6.212214	24690	25767	924-1259	1.000000	51.0
trnD-GUC-psbM_p5	trnD-GUC-psbM	5	GTTCGAGCCCCGTCAGTC	TCGTTTTGACTGACCGTTTTT	18	21	66.7	38.1	60.1	56.9	0.0	0.0	6.232772	24690	25767	923-1258	1.000000	51.0
trnG-UCC_p1	trnG-UCC	1	TCACATTAATGATACACGCATCA	TCTTGTTCGACAAATGGTCCA	23	21	34.8	42.9	56.4	57.8	22.2	0.0	5.773616	45602	46433	865-928	0.909091	46.1
trnG-UCC_p2	trnG-UCC	2	TTCACATTAATGATACACGCATCA	TCTTGTTCGACAAATGGTCCA	24	21	33.3	42.9	57.1	57.8	22.2	0.0	6.118571	45602	46433	866-929	0.909091	46.1
trnG-UCC_p3	trnG-UCC	3	CACATTAATGATACACGCATCA	TCTTGTTCGACAAATGGTCCA	22	21	36.4	42.9	55.0	57.8	22.2	0.0	6.201676	45602	46433	864-927	0.909091	46.1
trnG-UCC_p4	trnG-UCC	4	TCACATTAATGATACACGCATCA	CTTGTTCGACAAATGGTCCA	23	20	34.8	45.0	56.4	56.3	22.2	0.0	6.299672	45602	46433	864-927	0.909091	46.1
trnG-UCC_p5	trnG-UCC	5	TCACATTAATGATACACGCATCAT	TCTTGTTCGACAAATGGTCCA	24	21	33.3	42.9	56.9	57.8	22.2	0.0	6.309195	45602	46433	865-928	0.909091	46.1
trnG-UCC-trnS-GCU_p1	trnG-UCC-trnS-GCU	1	ACGAATCACACTTTTACCACT	TTATTCGTACCGAGGGTTCG	21	20	38.1	50.0	55.5	57.2	0.0	7.4	4.385723	46434	47606	1114-1458	1.000000	56.9
trnG-UCC-trnS-GCU_p2	trnG-UCC-trnS-GCU	2	ACGAATCACACTTTTACCACT	TTCGAATCCCTCTCTTTCCG	21	20	38.1	50.0	55.5	57.0	0.0	0.0	4.497369	46434	47606	1098-1442	1.000000	56.0
trnG-UCC-trnS-GCU_p3	trnG-UCC-trnS-GCU	3	AGAACGAATCACACTTTTACCAC	TTATTCGTACCGAGGGTTCG	23	20	39.1	50.0	57.3	57.2	0.0	7.4	4.518439	46434	47606	1117-1461	1.000000	55.9
trnG-UCC-trnS-GCU_p4	trnG-UCC-trnS-GCU	4	GAACGAATCACACTTTTACCACT	TTATTCGTACCGAGGGTTCG	23	20	39.1	50.0	57.3	57.2	0.0	7.4	4.518439	46434	47606	1116-1460	1.000000	55.9
trnG-UCC-trnS-GCU_p5	trnG-UCC-trnS-GCU	5	ACGAATCACACTTTTACCACT	GGGTTCGAATCCCTCTCTTT	21	20	38.1	50.0	55.5	56.9	0.0	17.2	4.618397	46434	47606	1101-1445	1.000000	55.1
trnS-GCU-trnL-UAA_p1	trnS-GCU-trnL-UAA	1	CAATCCGACGCTTTAGTCCA	CTACCAATTCCGCCATACCC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.112373	47695	48394	685-825	1.000000	91.1
trnS-GCU-trnL-UAA_p2	trnS-GCU-trnL-UAA	2	AATCCGACGCTTTAGTCCAC	CTACCAATTCCGCCATACCC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.113761	47695	48394	684-824	1.000000	91.1
trnS-GCU-trnL-UAA_p3	trnS-GCU-trnL-UAA	3	CAATCCGACGCTTTAGTCCA	AATCCAACCAAGTCCGTAGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.114007	47695	48394	707-847	1.000000	91.0
trnS-GCU-trnL-UAA_p4	trnS-GCU-trnL-UAA	4	AATCCGACGCTTTAGTCCAC	AATCCAACCAAGTCCGTAGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.115395	47695	48394	706-846	1.000000	91.0
trnS-GCU-trnL-UAA_p5	trnS-GCU-trnL-UAA	5	CAATCCGACGCTTTAGTCCA	CCGTAGCGTCTACCAATTCC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.155486	47695	48394	694-834	1.000000	90.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	22	20	36.4	50.0	56.1	57.8	0.0	0.0	4.083948	51457	52585	1092-1213	1.000000	59.3
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCATATTCGTGAAGCAGAAACA	GAAGGTCTACGGTTCGAGTC	22	20	36.4	55.0	56.1	57.5	0.0	0.0	4.427720	51457	52585	1091-1212	1.000000	56.6
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCATATTCGTGAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	22	20	36.4	55.0	56.1	58.7	0.0	0.0	4.570014	51457	52585	1080-1201	1.000000	55.4
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	21	20	38.1	50.0	54.6	57.8	0.0	0.0	4.585537	51457	52585	1091-1212	1.000000	55.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCATATTCGTGAAGCAGAAACAT	AGAAGGTCTACGGTTCGAGT	23	20	34.8	50.0	56.6	57.8	0.0	0.0	4.587896	51457	52585	1092-1213	1.000000	55.3
petL-petG_p1	petL-petG	1	TGGTCTGAGCAAGATACGAC	ACGAATAACCCAGCCAAAGT	20	20	50.0	45.0	57.1	57.4	7.1	0.0	1.520863	66985	67159	262-292	1.000000	77.5
petL-petG_p2	petL-petG	2	TTGGTCTGAGCAAGATACGAC	ACGAATAACCCAGCCAAAGT	21	20	47.6	45.0	57.8	57.4	17.5	0.0	1.811623	66985	67159	263-293	1.000000	75.2
petL-petG_p3	petL-petG	3	GGTCTGAGCAAGATACGACT	ACGAATAACCCAGCCAAAGT	20	20	50.0	45.0	56.8	57.4	1.3	0.0	1.816920	66985	67159	261-291	1.000000	75.2
petL-petG_p4	petL-petG	4	TGGTCTGAGCAAGATACGAC	ACGCAGTTACGAATAACCCA	20	20	50.0	45.0	57.1	57.3	7.1	0.0	1.688849	66985	67159	270-300	0.909091	72.2
petL-petG_p5	petL-petG	5	TGGTCTGAGCAAGATACGAC	AGGCCTAACACGATTCCAAA	20	20	50.0	45.0	57.1	57.1	7.1	1.3	1.812549	66985	67159	232-262	0.909091	71.4
rps12-clpP_p1	rps12-clpP	1	AGAGCGGGGGATTTTGTTAC	ATCGAAAATGCCGGGGATTT	20	20	50.0	45.0	57.9	58.2	0.0	19.4	0.348892	70805	70970	243-266	1.000000	87.8
rps12-clpP_p2	rps12-clpP	2	GAGCGGGGGATTTTGTTACA	ATCGAAAATGCCGGGGATTT	20	20	50.0	45.0	58.2	58.2	0.0	19.4	0.395636	70805	70970	242-265	1.000000	87.4
rps12-clpP_p3	rps12-clpP	3	AGAGCGGGGGATTTTGTTAC	GAAAATGCCGGGGATTTCAC	20	20	50.0	50.0	57.9	57.7	0.0	24.2	0.417954	70805	70970	240-263	1.000000	87.0
rps12-clpP_p4	rps12-clpP	4	GAGCGGGGGATTTTGTTACA	GAAAATGCCGGGGATTTCAC	20	20	50.0	50.0	58.2	57.7	0.0	24.2	0.464698	70805	70970	239-262	1.000000	86.6
rps12-clpP_p5	rps12-clpP	5	CACCCGAGTACATGTTCCTC	ATCGAAAATGCCGGGGATTT	20	20	55.0	45.0	57.7	58.2	2.3	19.4	0.519868	70805	70970	283-306	1.000000	85.8
ndhF_p1	ndhF	1	ATTTGGTGGTGGTGAACTCC	CTCACTCCGTTCTCCAACAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.076312	111966	114197	2922-2966	0.545455	68.0
ndhF_p2	ndhF	2	ATTTGGTGGTGGTGAACTCC	AACAGGCTAGTTCACAACCG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.125671	111966	114197	2907-2951	0.545455	67.6
ndhF_p3	ndhF	3	TGGTGAACTCCCGCTTACTA	CTCACTCCGTTCTCCAACAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.144201	111966	114197	2913-2957	0.545455	67.5
ndhF_p4	ndhF	4	GAACTCCCGCTTACTACTGC	CTCACTCCGTTCTCCAACAG	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.147789	111966	114197	2909-2953	0.545455	67.5
ndhF_p5	ndhF	5	ATTTGGTGGTGGTGAACTCC	AAGCAACTCACTCCGTTCTC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.160967	111966	114197	2928-2972	0.545455	67.3
ndhF-rpl32_p1	ndhF-rpl32	1	AAGGGGGACGAAAGGTATGA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.046697	114198	115856	983-2212	1.000000	91.6
ndhF-rpl32_p2	ndhF-rpl32	2	AAGGGGGACGAAAGGTATGA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048068	114198	115856	984-2213	1.000000	91.6
ndhF-rpl32_p3	ndhF-rpl32	3	ACGAAAGGTATGATCCACGC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071898	114198	115856	976-2205	1.000000	91.4
ndhF-rpl32_p4	ndhF-rpl32	4	CGAAAGGTATGATCCACGCA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	114198	115856	975-2204	1.000000	91.4
ndhF-rpl32_p5	ndhF-rpl32	5	AAGGGGGACGAAAGGTATGA	GGAGGAGGCAATTCGCAATA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.071549	114198	115856	909-2138	0.818182	84.2
ccsA-ndhD_p1	ccsA-ndhD	1	ATTCTGCAATTGTGGCTTCT	ATCCGGATTTCGTTCTCTCA	20	20	40.0	45.0	55.9	56.4	7.4	12.4	3.741746	117157	117411	415-647	1.000000	62.1
ccsA-ndhD_p2	ccsA-ndhD	2	ATTCTGCAATTGTGGCTTCT	TCCGGATTTCGTTCTCTCAT	20	20	40.0	45.0	55.9	56.4	7.4	0.0	3.741746	117157	117411	414-646	1.000000	62.1
ccsA-ndhD_p3	ccsA-ndhD	3	TCAAATTCTGCAATTGTGGCT	ATCCGGATTTCGTTCTCTCA	21	20	38.1	45.0	57.0	56.4	7.4	12.4	3.674354	117157	117411	419-651	0.909091	59.0
ccsA-ndhD_p4	ccsA-ndhD	4	TCAAATTCTGCAATTGTGGCT	TCCGGATTTCGTTCTCTCAT	21	20	38.1	45.0	57.0	56.4	7.4	0.0	3.674354	117157	117411	418-650	0.909091	59.0
ccsA-ndhD_p5	ccsA-ndhD	5	TCAAATTCTGCAATTGTGGCT	TCCGGATTTCGTTCTCTCATT	21	21	38.1	42.9	57.0	57.1	7.4	0.0	3.921210	117157	117411	418-650	0.909091	57.0
ndhE-ndhG_p1	ndhE-ndhG	1	TCAAGAAATCGGTTGGGCAA	TGGACTGTTGGAGATGGAGT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.041734	119855	120114	1234-1271	1.000000	89.4
ndhE-ndhG_p2	ndhE-ndhG	2	TCAAGAAATCGGTTGGGCAA	CCATTTTGTAGCTGCTGCAC	20	20	45.0	50.0	58.0	58.0	0.0	20.1	0.025009	119855	120114	1346-1383	1.000000	89.1
ndhE-ndhG_p3	ndhE-ndhG	3	CGTCCCAGGGTATCATCTCT	TGGACTGTTGGAGATGGAGT	20	20	55.0	50.0	58.0	58.0	5.0	0.0	0.032533	119855	120114	1141-1178	0.818182	80.5
ndhE-ndhG_p4	ndhE-ndhG	4	CGTCCCAGGGTATCATCTCT	CCATTTTGTAGCTGCTGCAC	20	20	55.0	50.0	58.0	58.0	5.0	20.1	0.015807	119855	120114	1253-1290	0.818182	80.3
ndhE-ndhG_p5	ndhE-ndhG	5	CGTCCCAGGGTATCATCTCT	GTGGGAAGTCTAGGAGTGGT	20	20	55.0	55.0	58.0	58.1	5.0	0.0	0.062525	119855	120114	1357-1394	0.818182	79.5
ndhG-ndhI_p1	ndhG-ndhI	1	ATTGGTCCAGGCAAATCCAT	TTCAAGAACAATTACCAATTACT	20	23	45.0	26.1	57.4	52.3	0.0	0.0	9.277375	120649	121022	419-535	1.000000	52.0
ndhG-ndhI_p2	ndhG-ndhI	2	ATTGGTCCAGGCAAATCCAT	GATTCAAGAACAATTACCAATTACT	20	25	45.0	28.0	57.4	54.1	0.0	0.0	9.433553	120649	121022	421-537	1.000000	52.0
ndhG-ndhI_p3	ndhG-ndhI	3	ATTGGTCCAGGCAAATCCAT	ATTCAAGAACAATTACCAATTACT	20	24	45.0	25.0	57.4	52.9	0.0	0.0	9.643930	120649	121022	420-536	1.000000	52.0
ndhG-ndhI_p4	ndhG-ndhI	4	ATTGGTCCAGGCAAATCCAT	GATTCAAGAACAATTACCAATTAC	20	24	45.0	29.2	57.4	52.7	0.0	0.0	9.806570	120649	121022	421-537	1.000000	52.0
ndhG-ndhI_p5	ndhG-ndhI	5	TCATGTATTGGTCCAGGCAA	TTCAAGAACAATTACCAATTACT	20	23	45.0	26.1	56.8	52.3	0.0	0.0	9.913369	120649	121022	425-541	1.000000	52.0
ycf1_p1	ycf1	1	ATCCCCGATTAGTTTGGTGC	AGGCCCAAAGGATTCCCTAT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.125731	126477	126596	1077-1113	0.909091	84.1
ycf1_p2	ycf1	2	ATCCCCGATTAGTTTGGTGC	GGGGCCAGTGCTAACATTAA	20	20	50.0	50.0	57.9	57.9	0.0	4.4	0.190592	126477	126596	871-901	0.909091	83.7
ycf1_p3	ycf1	3	TCCCATTTTCATATCGCGCA	GGGGCCAGTGCTAACATTAA	20	20	45.0	50.0	58.0	57.9	8.7	4.4	0.177393	126477	126596	937-967	0.818182	79.5
ycf1_p4	ycf1	4	TCCCATTTTCATATCGCGCA	AGGCCCAAAGGATTCCCTAT	20	20	45.0	50.0	58.0	58.1	8.7	0.0	0.112532	126477	126596	1143-1179	0.818182	79.3
ycf1_p5	ycf1	5	TCCCATTTTCATATCGCGCA	AATGAGGCCCAAAGGATTCC	20	20	45.0	50.0	58.0	57.8	8.7	0.0	0.204941	126477	126596	1147-1183	0.818182	78.5