Hotspot

ycf1

IRb · 107595-110440 · 2846 bp

Back to Anemonastrum

Pi 0.032

MarkerSeek score 55.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 2846
variable_sites: 153
pi: 0.032
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCAATAGAAAAGATAAAAAGGAGGT	GGAGGCCGAATCTCTTCTTA	30.8	50.0	57.3	56.4	2990 bp	0.25	10.0
ycf1_p2	AGAAAAGATAAAAAGGAGGTTTGTG	GGAGGCCGAATCTCTTCTTA	32.0	50.0	56.2	56.4	2984 bp	0.25	10.0
ycf1_p3	TGCAATAGAAAAGATAAAAAGGAGGTT	GGAGGCCGAATCTCTTCTTA	29.6	50.0	57.9	56.4	2990 bp	0.25	10.0
ycf1_p4	TTGCAATAGAAAAGATAAAAAGGAGGT	GGAGGCCGAATCTCTTCTTA	29.6	50.0	57.9	56.4	2991 bp	0.25	10.0
ycf1_p5	GAAAAGATAAAAAGGAGGTTTGTG	GGAGGCCGAATCTCTTCTTA	33.3	50.0	54.9	56.4	2983 bp	0.25	10.0