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psbE-petL

LSC · 63253-64658 · 1406 bp

Back to Anemonastrum

Pi 0.0543

MarkerSeek score 83.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1406
variable_sites: 125
pi: 0.0543
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCCAGTAGAAAACCGAAATAACT	50.0	39.1	57.9	57.1	1352-1548 bp	1.0	59.4
psbE-petL_p2	ACGAATAACCAACCCGCAAT	GCCAGTAGAAAACCGAAATAACT	45.0	39.1	58.2	57.1	1361-1557 bp	1.0	58.4
psbE-petL_p3	TGCTGACGAATAACCAACCC	GCCAGTAGAAAACCGAAATAACT	50.0	39.1	58.2	57.1	1366-1562 bp	1.0	58.3
psbE-petL_p4	CGAATAACCAACCCGCAATG	GCCAGTAGAAAACCGAAATAACT	50.0	39.1	57.8	57.1	1360-1556 bp	1.0	58.3
psbE-petL_p5	ACCAACCCGCAATGAATAGG	GCCAGTAGAAAACCGAAATAACT	50.0	39.1	58.2	57.1	1354-1550 bp	1.0	57.9