Hotspot

ycf4-cemA

LSC · 59606-60197 · 592 bp

Back to Anemonastrum

Pi 0.0561

MarkerSeek score 80.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 62
pi: 0.0561
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GGTATTTATTCCCGGCGTGT	GTATCGGGGTAGGGGTACTC	50.0	60.0	58.0	58.1	987-999 bp	1.0	85.8
ycf4-cemA_p2	AACAAAAGGCTGCGGAATTG	GTATCGGGGTAGGGGTACTC	45.0	60.0	57.8	58.1	890-902 bp	1.0	85.5
ycf4-cemA_p3	CGGGTCTGTTCATTAGCTCC	GTATCGGGGTAGGGGTACTC	55.0	60.0	58.1	58.1	1175-1187 bp	1.0	84.8
ycf4-cemA_p4	AGTAATTTCTGCTGGGCCTG	GTATCGGGGTAGGGGTACTC	50.0	60.0	57.9	58.1	1332-1344 bp	1.0	83.7
ycf4-cemA_p5	GCTGGGCCTGTATCCTTTTT	GTATCGGGGTAGGGGTACTC	50.0	60.0	58.1	58.1	1322-1334 bp	1.0	83.6