Hotspot

trnF-GAA-ndhJ

LSC · 47322-47840 · 519 bp

Back to Anemonastrum

Pi 0.0652
MarkerSeek score 82.30
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
519
variable_sites
56
pi
0.0652
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.87
markerseek_score
82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA GCCCCAAACTTCTATGAAATCC 50.0 45.5 58.1 57.3 589-774 bp 1.0 68.6
trnF-GAA-ndhJ_p2 AATCCTCGTGTCACCAGTTC GCCCCAAACTTCTATGAAATCC 50.0 45.5 57.5 57.3 592-777 bp 1.0 65.3
trnF-GAA-ndhJ_p3 AAGAGTCGGGATAGCTCAGT GCCCCAAACTTCTATGAAATCC 50.0 45.5 57.6 57.3 632-817 bp 1.0 65.1
trnF-GAA-ndhJ_p4 AGAGTCGGGATAGCTCAGTT GCCCCAAACTTCTATGAAATCC 50.0 45.5 57.6 57.3 631-816 bp 1.0 65.1
trnF-GAA-ndhJ_p5 TCGGGATAGCTCAGTTGGTA GCCCCAAACTTCTATGAAATCC 50.0 45.5 57.6 57.3 627-812 bp 1.0 65.1