Hotspot

trnG-GCC-trnS-GCU

LSC · 45199-45588 · 390 bp

Back to Anemonastrum

Pi 0.269
MarkerSeek score 80.10
Primer available yes
Label trnG-GCC-trnS-GCU

Feature Metadata

label_name
trnG-GCC-trnS-GCU
feature_type
igs
region
LSC
length_bp
390
variable_sites
167
pi
0.269
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnS-GCU_p1 ACGAATCACACTTTTACCACT TTCGAATCCCTCTCTTTCCG 38.1 50.0 55.5 57.0 390-1602 bp 1.0 56.0
trnG-GCC-trnS-GCU_p2 ACGAATCACACTTTTACCACT GGGTTCGAATCCCTCTCTTT 38.1 50.0 55.5 56.9 393-1605 bp 1.0 55.1
trnG-GCC-trnS-GCU_p3 ACGAATCACACTTTTACCACT GGGTTCGAATCCCTCTCTTTC 38.1 52.4 55.5 58.1 393-1605 bp 1.0 55.0
trnG-GCC-trnS-GCU_p4 ACGAATCACACTTTTACCACT GGTTCGAATCCCTCTCTTTCC 38.1 52.4 55.5 58.1 392-1604 bp 1.0 55.0
trnG-GCC-trnS-GCU_p5 GAACGAATCACACTTTTACCACT TTCGAATCCCTCTCTTTCCG 39.1 50.0 57.3 57.0 392-1604 bp 1.0 55.0