Hotspot

trnR-UCU-trnG-GCC

LSC · 44266-44424 · 159 bp

Back to Anemonastrum

Pi 0.073

MarkerSeek score 81.30

Primer available yes

Label trnR-UCU-trnG-GCC

Feature Metadata

label_name: trnR-UCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 159
variable_sites: 21
pi: 0.073
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-GCC_p1	TGGAAACAAATTGCGTCCAA	AGCCTTCCAAGCTAACGATG	40.0	50.0	56.7	58.0	281-286 bp	1.0	77.3
trnR-UCU-trnG-GCC_p2	TGGAAACAAATTGCGTCCAA	CCTAGCCTTCCAAGCTAACG	40.0	55.0	56.7	58.1	284-289 bp	1.0	77.0
trnR-UCU-trnG-GCC_p3	TGGAAACAAATTGCGTCCAA	CCCTAGCCTTCCAAGCTAAC	40.0	55.0	56.7	57.7	285-290 bp	1.0	74.8
trnR-UCU-trnG-GCC_p4	TGGAAACAAATTGCGTCCAA	CTAACGATGCGGGTTCGATT	40.0	50.0	56.7	58.4	270-275 bp	1.0	74.4
trnR-UCU-trnG-GCC_p5	TGGAAACAAATTGCGTCCAA	GGCGTCGACTATAACCCCTA	40.0	55.0	56.7	58.4	300-305 bp	1.0	74.0