Hotspot

trnC-GCA-rpoB

LSC · 25962-27127 · 1166 bp

Back to Anemonastrum

Pi 0.0467

MarkerSeek score 80.70

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1166
variable_sites: 99
pi: 0.0467
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	TTAAAGCAGCCCAAGCAAGA	CCGCCTACACAAGCAAATTG	45.0	50.0	58.0	58.0	2332-2366 bp	1.0	86.2
trnC-GCA-rpoB_p2	CATGTGAATTATGCCCCCGA	CCGCCTACACAAGCAAATTG	50.0	50.0	58.0	58.0	2301-2335 bp	1.0	86.1
trnC-GCA-rpoB_p3	CCGGATTTGAACTGGGGAAA	CCGCCTACACAAGCAAATTG	50.0	50.0	58.1	58.0	1915-1955 bp	1.0	85.8
trnC-GCA-rpoB_p4	TTAAAGCAGCCCAAGCAAGA	GACTCCGAAAATACCGGGTC	45.0	55.0	58.0	58.1	2376 bp	0.25	49.4
trnC-GCA-rpoB_p5	CATGTGAATTATGCCCCCGA	GACTCCGAAAATACCGGGTC	50.0	55.0	58.0	58.1	2345 bp	0.25	49.3