Hotspot

psbM-petN

LSC · 24309-25420 · 1112 bp

Back to Anemonastrum

Pi 0.0661

MarkerSeek score 84.00

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 1112
variable_sites: 137
pi: 0.0661
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAAACGGTCAGCCAAAACG	TCTTGCTTGGGCTGCTTTAA	45.0	45.0	58.1	58.0	1183-1213 bp	1.0	90.9
psbM-petN_p2	AAAAACGGTCAGCCAAAACG	TCGGGGGCATAATTCACATG	45.0	50.0	58.1	58.0	1214-1244 bp	1.0	90.7
psbM-petN_p3	ACGGTCAGCCAAAACGATTA	TCTTGCTTGGGCTGCTTTAA	45.0	45.0	57.8	58.0	1179-1209 bp	1.0	90.2
psbM-petN_p4	ACGGTCAGCCAAAACGATTA	TCGGGGGCATAATTCACATG	45.0	50.0	57.8	58.0	1210-1240 bp	1.0	90.0
psbM-petN_p5	AAAAACGGTCAGCCAAAACG	TTTCCCCAGTTCAAATCCGG	45.0	50.0	58.1	58.1	1600-1636 bp	1.0	89.5