Hotspot

trnT-GGU-trnE-UUC

LSC · 21701-22341 · 641 bp

Back to Anemonastrum

Pi 0.0549

MarkerSeek score 87.60

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 641
variable_sites: 61
pi: 0.0549
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 87.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	TTTCGTAGTACCCTACCCCC	50.0	55.0	58.0	57.8	644-782 bp	1.0	89.3
trnT-GGU-trnE-UUC_p2	GAACCGATGACTTACGCCTT	TGTCCTGAACCACTAGACGA	50.0	50.0	58.0	57.7	585-723 bp	1.0	88.9
trnT-GGU-trnE-UUC_p3	TACCGCTGAGTTAAAAGGGC	TTTCGTAGTACCCTACCCCC	50.0	55.0	57.9	57.8	609-747 bp	1.0	88.8
trnT-GGU-trnE-UUC_p4	GAACCGATGACTTACGCCTT	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	58.0	58.3	606-744 bp	1.0	88.8
trnT-GGU-trnE-UUC_p5	TGGAAAAAGCCCCTTATCGG	TTTCGTAGTACCCTACCCCC	50.0	55.0	57.9	57.8	668-806 bp	1.0	88.0