Hotspot

psbD-trnT-GGU

LSC · 20469-21628 · 1160 bp

Back to Anemonastrum

Pi 0.0622

MarkerSeek score 87.60

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1160
variable_sites: 123
pi: 0.0622
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 87.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	TCTCCGTAACCAGTCATCCA	GCCCTTTTAACTCAGCGGTA	50.0	50.0	57.8	57.9	1297-1366 bp	1.0	88.8
psbD-trnT-GGU_p2	TCTCCGTAACCAGTCATCCA	GGCGTAAGTCATCGGTTCAA	50.0	50.0	57.8	58.3	1260-1329 bp	1.0	87.6
psbD-trnT-GGU_p3	GGTCTCTCCGTAACCAGTCA	GCCCTTTTAACTCAGCGGTA	55.0	50.0	58.5	57.9	1301-1370 bp	1.0	87.0
psbD-trnT-GGU_p4	GGTCTCTCCGTAACCAGTCA	GGCGTAAGTCATCGGTTCAA	55.0	50.0	58.5	58.3	1264-1333 bp	1.0	85.7
psbD-trnT-GGU_p5	TCTCCGTAACCAGTCATCCA	GCCATGGTAAGGCGTAAGTC	50.0	55.0	57.8	58.7	1270-1339 bp	1.0	84.2