Hotspot

ycf3-psaA

LSC · 10679-11471 · 793 bp

Back to Anemonastrum

Pi 0.0606

MarkerSeek score 86.80

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 793
variable_sites: 80
pi: 0.0606
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 86.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	TTGGTTGAAGATCACGAGGC	GCCCATTCCTCGAAAGAAGT	50.0	50.0	58.2	57.9	864-904 bp	1.0	88.9
ycf3-psaA_p2	TTGGTTGAAGATCACGAGGC	GAGAAATGACCTGGTCTGGC	50.0	55.0	58.2	58.3	882-922 bp	1.0	87.6
ycf3-psaA_p3	TCACGAGGCGGTTTGAATAA	GCCCATTCCTCGAAAGAAGT	45.0	50.0	57.5	57.9	853-893 bp	1.0	86.8
ycf3-psaA_p4	TCACGAGGCGGTTTGAATAA	GAGAAATGACCTGGTCTGGC	45.0	55.0	57.5	58.3	871-911 bp	1.0	85.5
ycf3-psaA_p5	GATCACGAGGCGGTTTGAAT	GCCCATTCCTCGAAAGAAGT	50.0	50.0	58.6	57.9	855-895 bp	1.0	85.4