Hotspot

psbK-psbI

LSC · 7094-7508 · 415 bp

Back to Anemonastrum

Pi 0.0612

MarkerSeek score 80.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 415
variable_sites: 48
pi: 0.0612
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCGGCTGTAAGTTTTCG	TCTTCACGTCCAGGATTACG	50.0	50.0	58.0	57.1	546-573 bp	1.0	81.4
psbK-psbI_p2	AGCGGCTGTAAGTTTTCGAT	TCTTCACGTCCAGGATTACG	45.0	50.0	57.9	57.1	544-571 bp	1.0	81.1
psbK-psbI_p3	AAGCGGCTGTAAGTTTTCGA	TCTTCACGTCCAGGATTACG	45.0	50.0	58.1	57.1	545-572 bp	1.0	80.8
psbK-psbI_p4	GCAAGCGGCTGTAAGTTTTC	TCTTCACGTCCAGGATTACG	50.0	50.0	58.3	57.1	547-574 bp	1.0	79.3
psbK-psbI_p5	CAAGCGGCTGTAAGTTTTCG	TTCTTCACGTCCAGGATTACG	50.0	47.6	58.0	57.8	547-574 bp	1.0	79.0